KMID : 0382619880080010423
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Hanyang Journal of Medicine 1988 Volume.8 No. 1 p.423 ~ p.439
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Study for the Cellular Fatty Acid Profiles of Genus Staphylococcus Using Gas Chromatography
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ÑÑÐñÔÔ/Kim, Ki Dong
ÑÑõðêª/ùàËíñº/ûóâ×ýï/Kim, Choon Won/Hahm, Kun Ju/Hong, Sook Hee
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Abstract
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Whole-cell fatty acid methylester profiles of the Genus Staphylococcus were deter-mined by gas chromatogrphy with application of the flexible, fused-silica capillary column for increased resolution.
The results of the study were as follows:
1. The major cellular fatty acids were C15:0 Iso, C15:0 Anteiso, C17:0 Iso, C17:0 Anteiso, C18:0, C20:0.
2. Staphylococcus aureus-GC subgroup A contained relatively large amount of C15:0 Anteiso and C17:0 Anteiso than subgroup B.
3. Staphylococcus aureus-GC subgroup B contained relatively large amount of C20:0 than subgroup A.
4. The cellular fatty acid of the Genus Staphylococcus were similar, but characterized by relatively large amounts and small amounts as follows:
1) Staphylococcus cohnii contained large amounts of C15:0 Iso, C15:0 Anteiso, C17:0 Iso, C17:0 Anteiso and contained small amounts of C20:0 than Staphylococcus aureus.
2) Staphylococcus haemolyticus contained large amounts of C15:0 Anteiso, C17:0 !ht Iso, C17:0 Anteiso and contained small amounts of C20:0 than Staphylococcus aureus.
3) Staphylococcus simulans contained large amounts of C15:0 Iso, C15:0 Anteiso, C17:0 Iso, C17:6 Anteiso and contained small amounts of C20:0 than Staphylococcus aureus.
4) Staphylococcus epidermidis contained large amounts of C15:0 Anteiso than Staphylococcus aureus.
5) Staphylococcus warneri contained large amounts of C15:0 Anteiso than Staphylococcus aureus.
6) Staphylococcus caprae contained large amounts of C15:0 Anteiso and contained small amounts of C20:0 than Staphylococcus aureus.
In conclusion, this study provided useful information for -rapid detection and identification of several bacterial groups or species.
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KEYWORD
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